Before phosphorylase can resume catabolism, debranching enzymes perform two functions:
2.
Pullulanase is also known as pullulan-6-glucanohydrolase ( Debranching enzyme ).
3.
Together with phosphorylase, glycogen debranching enzymes function in glycogen breakdown and glucose mobilization.
4.
Proteins that catalyze both functions are referred to as glycogen debranching enzymes ( GDEs ).
5.
Glycogen debranching enzymes assist phosphorylase, the primary enzyme involved in glycogen breakdown, mobilize glycogen stores.
6.
The gene is responsible for creating glycogen debranching enzyme, which in turn helps in glycogen decomposition.
7.
In some literature, an enzyme capable only of glucosidase is referred to as a " debranching enzyme ".
8.
When glucosyltransferase and glucosidase are catalyzed by distinct enzymes, " glycogen debranching enzyme " usually refers to the glucosidase enzyme.
9.
The enzymes used in the saccharification step are the amylolytic enzyme, beta-amylase ( usually derived from " Bacillus species " ) and the debranching enzyme, pullulanase ( derived from " Aerobacter species " ).
10.
In " E . coli ", Glucose transfer is performed by 4-alpha-glucanotransferase, a 78.5 kDa protein coded for by the gene malQ . A second protein, referred to as debranching enzyme, performs ?-1, 6-glucose cleavage.
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